Recently started projects (2022-)
Druggability of mechanisms in rare diseases associated with Ras signalling pathways
- EURAS – European Network for Developmental Rasopathies (consortium website)
- Identifying targetable defects of SynGAP1 missense variants (Courtney MJ & Li L-L)
Signalling dynamics and encoding
- Temporally-encoded intracellular signalling and its impact on single cell integration into synaptically active neuronal circuits
Synapse biology
- The synaptic life cycle regulated by disease mechanisms (Li L-L)
Earlier projects
Organisation of Signalling Pathways and Roles in Disorders of the Nervous System
- NOS1AP/CAPON: mechanisms of action on NMDA receptor signalling through nNOS (review)
- NOS1AP/CAPON : contribution to diseases and disorders (hypoxia-ischaemia, neuropathic pain, schizophrenia, neurodegenerative disorders)
Optogenetic and Optopharmacological tools and techniques for precise control of cell signalling pathways
- OptoJNKi and Optop38i, new tools for precise spatiotemporal control of JNK and p38 pathways (see www.nature.com/articles/ncomms15017, press release, plasmids)
- LOV2FRET – a single solution to monitor optogenetic actuation in situ and increase light-sensitivity to limit long-term phototoxicity (see www.nature.com/articles/s41467-020-18816, blogpost, data, protocol, plasmids)
- Higher throughput in microscopy-based assays and in situ quantification of cell signalling kinetics with a slow-relaxing form of OptoJNKi (OptoJNKi-sr; see www.nature.com/articles/s41467-020-18816)
- Design strategies for rapid generation of optically regulated inhibitors – extending the toolbox
Development and Application of High-Content Analysis and High-Throughput Microscopy tools and methods
- An automated single-cell biology pipeline for analysis of signalling circuit dynamics in interconnected cell networks
- RNAi-based identification of RasGEFs counteracting neuronal SynGAP1 function
- Workshops: check back later for the next workshop (last workshop was 19-20 June 2017)
- Provision of high-throughput microscopy and liquid handling infrastructure and services for biological research, chemical biology and early-stage drug discovery (Screening unit)