The instrument has a dual-camera setup with a colour camera and monochromatic camera for fluorescence imaging. It has two white light sources for brightfield imaging, a ring light from above and light below the sample. Fluorescence optical sectioning is possible via structured illumination using the ApoTome.2.

    Fluorophore selection guide

    Reservation System: Zeiss AxioZoom.V16

    Location: BioCity, 5th floor, room Dunkel

    0.5x PlanApo Z
    • Numerical Aperture: 0.125
    • Working Distance: 114mm
    • Magnification range: 3.5x-56x
    • Field of view range: 66-4mm
    • Apotome compatible: No
    1.0x PlanApo Z
    • Numerical Aperture: 0.125
    • Working Distance: 56mm
    • Magnification range: 7x-112x
    • Field of view range: 33-2mm
    • Apotome: Yes from 40x-112x
    Two White light sources :
    • Above: CL 9000 LED CAN ring light
    • Below: visiLED MC1000
    Fluorescence Excitation:
    • HXP 200C mercury lamp
    DAPI - filter set 49
    Excitation: G365nm
    Emission: BP 445/50nm

    Alexa 488 - filter set 38 HE
    Excitation: BP 470/40nm
    Emission: BP 525/50nm
    Alexa 568 - filter set 45
    Excitation: BP 560/40nm
    Emission: BP 630/75nm

    Alexa 647 - filter set 50
    Excitation: BP 640/30nm
    Emission: 690/50nm

    With structured illumination (SIM), you know that only the focal plane appears in your image: ApoTome.2 recognizes the magnification and moves the appropriate grid into the beampath. The system then calculates your optical section from three (or more) images with different grid positions. This removes scattered out-of-focus light from your images, even in thicker specimens.

    AxioCam 105 Colour
    2560 x 1920 pixels
    • Zen Pro